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Image Search Results
Journal: Molecular medicine reports
Article Title: CXC195 induces apoptosis and endoplastic reticulum stress in human hepatocellular carcinoma cells by inhibiting the PI3K/Akt/mTOR signaling pathway.
doi: 10.3892/mmr.2015.4479
Figure Lengend Snippet: Figure 2. Effects of CXC195 on the proliferation of HepG2 cells. (A) Cell cycle distribution was examined using flow cytometry. (B and C) Protein expres sion levels of PCNA, Ki67, CDK4, cyclin D1, p21, p27 and p53 were determined using western blot analysis. GAPDH was used to confirm equal protein loading. Data are presented as the mean ± standard deviation (n=6). *P<0.05 and **P<0.01, vs. control. PCNA, proliferating cell nuclear antigen; CDK4, cyclin‑dependent kinase 4; Con, control.
Article Snippet: The membrane was blocked with 5% skimmed milk and incubated with PBS containing Tween 20 and the following primary antibodies at 1:500 dilution:
Techniques: Flow Cytometry, Western Blot, Standard Deviation, Control